| Subdivision | From | To |
|---|---|---|
| Alvinston | 0.00 (Glencoe) |
10.85 (Alvinston) |
| Beach | 0.0 (Stoney Creek) |
7.8 (Burlington) |
| Burford | 0.00 (Brantford) |
35.83 (Tillsonburg Junction) |
| Dundas | 0.00 (Bayview) |
78.21 (London) |
| Dunnville | 0.1 (Fort Erie) |
75.4 (Brantford) |
| Grimsby | 2.33 (Clifton Junction Switch) |
43.7 (Hamilton) |
| Hagersville | 0.0 (Hamilton) |
32.7 (Jarvis) |
| Hickson | 0.0 (Woodstock) |
8.87 (Hickson) |
| Humberstone | 0.00 (Port Colborne) |
4.54 (Welland Junction) |
| Longwood | 0.00 (Komoka) |
20.38 (Glencoe) |
| Oakville | 9.42 (Toronto) |
39.29 (Hamilton) |
| Otterville | 0.00 (Otterville) |
7.55 (Burgessville) |
| Petrolia | 0.00 (Petrolia) |
4.68 (Petrolia Junction) |
| Point Edward | 0.00 (East Summit) |
4.72 (Point Edward) |
| Port Rowan | 0.00 (Simcoe) |
16.92 (Port Rowan) |
| St. Clair Tunnel | 0.0 (Sarnia) |
3.1 (Port Huron, MI) |
| Simcoe | 0.00 (Port Dover) |
9.24 (Simcoe Junction) |
| Strathroy | 0.00 (London) |
58.89 (Sarnia) |
| Thorold | 0.0 (Port Robinson) |
7.9 (Merriton) |